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flu-4 am  (Beyotime)


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    Beyotime flu-4 am
    Flu 4 Am, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flu-4+am/flu+4+am/pm37531596-284-23-25
    Average 90 stars, based on 1 article reviews
    flu-4 am - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

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    Article Snippet: Bone marrow mesenchymal stem cells (BMSCs) are suggested as candidates for neurodegeneration therapy by autologous stem cells to overcome the lack of neural stem cells in adults.. However, the differentiation of BMSCs into functional neurons is a major challenge for neurotherapy.. Herein, a methodology has been proposed to induce functional neuronal differentiation of BMSCs on a conductive three-dimensional graphene framework (GFs) combined with a rotating magnetic field.

    Article Title: Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.
    Article Snippet: X. Lu, C. Sun, Z. Feng, H. Gao, S. Hu, M. Dong, J. Wang, W. Zhou, N. Ren, H. Liu Institute for Advanced Interdisciplinary Research (iAIR) University of Jinan Jinan 250022, China E-mail: ifc_sunch@ujn.edu.cn L. Chen, H. Zhou Department of Orthopaedics Shandong University Centre for Orthopaedics, Qilu Hospital Cheeloo College of Medicine Shandong University Jinan, Shandong 250012, China E-mail: zhouhengxing@sdu.edu.cn H. Liu State Key Laboratory of Crystal Materials Shandong University Jinan 250100, China E-mail: hongliu@sdu.edu.cn



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    flu-4/am #s1060  (Beyotime)
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    STIM1 knockdown in <t>LS174T</t> cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.
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    STIM1 knockdown in <t>LS174T</t> cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.
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    STIM1 knockdown in LS174T cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: STIM1 Deficiency In Intestinal Epithelium Attenuates Colonic Inflammation and Tumorigenesis by Reducing ER Stress of Goblet Cells

    doi: 10.1016/j.jcmgh.2022.03.007

    Figure Lengend Snippet: STIM1 knockdown in LS174T cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.

    Article Snippet: LS174T cells seeded in 35-mm diameter, glass-bottom dishes (#D35-20-0-N; Cellvis, Mountin View, CA) were loaded with 5 μmol/L Flu-4/AM (#S1060; Beyotime, Shanghai, China) for 30 minutes in Dulbecco’s modified Eagle medium with 10% FBS at room temperature.

    Techniques: Knockdown, Immunostaining, Fluorescence, Control