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flu-4 am  (Beyotime)


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    Beyotime flu-4 am
    Flu 4 Am, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flu-4+am/pm37531596-284-23-25?v=Beyotime
    Average 90 stars, based on 1 article reviews
    flu-4 am - by Bioz Stars, 2026-08
    90/100 stars

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    STIM1 knockdown in <t>LS174T</t> cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.
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    STIM1 knockdown in <t>LS174T</t> cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.
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    STIM1 knockdown in LS174T cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: STIM1 Deficiency In Intestinal Epithelium Attenuates Colonic Inflammation and Tumorigenesis by Reducing ER Stress of Goblet Cells

    doi: 10.1016/j.jcmgh.2022.03.007

    Figure Lengend Snippet: STIM1 knockdown in LS174T cells alleviates ER stress and prolonged ER stress–induced apoptosis by reducing Ca 2+ overload. LS174T cells were treated with 10 μg/mL Tm or 5 μmol/L TG for 6 hours, 12 hours, or 24 hours with or without STIM1 knockdown. ( A ) ER stress was examined by WB at 12 hours. ( B ) ER stress was quantified by qPCR at 6 hours. ( C and D ) Apoptosis was examined by WB and immunostaining for C-Caspase 3 at 24 hours. Scale bars : 200 μm. ( E ) Tm- or ( F ) TG-induced intracellular Ca 2+ concentrations were measured by Fluo-4 AM in 20 minutes ( left ), and representative changes in intracellular Ca 2+ fluorescence intensity at 15 minutes ( right ) in LS174T cells with or without STIM1 knockdown were shown (n = 40 cells per group). Results are presented as the means ± SEM. ∗∗∗∗ P < .0001, ∗∗∗ P < .001, ∗∗ P < .01, and ∗ P < .05; NS at P > .05. DAPI, 4′,6-diamidino-2-phenylindole; F/Fbase, Ca 2+ fluorescence intensity / base of Ca 2+ fluorescence intensity; siCtrl, siRNA-Control; siSTIM1, siRNA-STIM1.

    Article Snippet: LS174T cells seeded in 35-mm diameter, glass-bottom dishes (#D35-20-0-N; Cellvis, Mountin View, CA) were loaded with 5 μmol/L Flu-4/AM (#S1060; Beyotime, Shanghai, China) for 30 minutes in Dulbecco’s modified Eagle medium with 10% FBS at room temperature.

    Techniques: Knockdown, Immunostaining, Fluorescence, Control